Novel MOA: first and only ARV that inhibits HIV-1 attachment^1,2

RUKOBIA is the only therapy that directly targets gp120 preventing attachment to protect CD4⁺ T-cells^1,3

VIDEO: Background music starts; shortly after the headline and subhead appear, the glow behind “CD4” animates in.

SUPER: Look CD4WARD: Highlighting the Critical Importance of CD4+ in HIV

VIDEO: CD4+ T-cells visible

VO: CD4 T-cells play an essential role in immune response and control of chronic viral infections.

VIDEO: HIV-1 floats into frame VO: To initiate the viral replication cycle, HIV-1 virions must attach to target CD4 T-cells.

VIDEO: Camera zooms in closer to see gp proteins on HIV-1

VO: This attachment is mediated by viral envelope proteins comprised of gp120 gp41 subunits.

VIDEO: Zoom in on the gp120/gp41 receptors; the receptor transitions between open and closed states

VO: The gp120 gp41 trimer transitions between an open and closed state but CD4 attachment can only occur when the gp120 gp41 trimer is in the open state.

VIDEO: HIV-1 approaches the T-cell membrane; gp120 approaches and binds to the CD4 molecules; gp120 binds to CD4 and HIV starts sinking down and rippling into the T-cell membrane

VO: Binding of the trimer to the CD4 receptor results in a series of conformational changes that enable binding of cell surface co-receptors, CCR5 or CXCR4.

VIDEO: Camera pulls back as we see HIV-1 fusing with the T-cell, sinking down through the membrane, and creating a disruptive aqua-like ripple effect that moves across the surface

VO: Co-receptor binding causes further structural rearrangements that drive fusion of the membrane and allows viral replication to begin.

VIDEO: Close-up of RUKOBIA drug molecule; RUKOBIA drug molecules float toward the CD4+ T-cell with HIV-1 virions circulating

VO: RUKOBIA (fostemsavir) is the first attachment inhibitor with a first-in-class mechanism of action that blocks HIV-1 from interacting with host CD4 cells.

VIDEO: Drug molecule approaches HIV that is heading towards T-cell; drug molecule intercepts HIV by binding to gp120 on the envelope of HIV-1; RUKOBIA binds to gp120, which initiates a “frosted glass” effect that spreads across gp120 and then continues over HIV virion

VO: At a granular level, temsavir, the active moiety of RUKOBIA, binds to the gp120 subunit within the HIV-1 envelope. This locks the gp120 gp41 trimer in the closed state.

VIDEO: “Frosted glass” effect spreads across HIV to represent that it’s suspended in the closed state and cannot bind to CD4; gp120 is unable to bind and HIV floats away

VO: This action selectively inhibits any interaction between the HIV-1 virion and cellular CD4 receptors.

VIDEO: RUKOBIA drug molecules approach HIV, and we see subsequent frosted glass effect spreading over them as result of binding to gp120s; HIV virions with the frosted glass effect

VIDEO: RUKOBIA rotates and sparkles in space

VO: This novel mechanism of action makes RUKOBIA the only ARV to prevent HIV-1 from attaching to CD4 cells.

VIDEO: Camera pulls back through a field of RUKOBIA molecules

VO: There is an unmet need to optimize an effective regimen for heavily treatment-experienced people living with HIV failing their current ARV regimen—whether due to resistance, intolerance, or other safety considerations.

VIDEO: Headline fades into frame with RUKOBIA molecules floating in space; the “CD4” text is animated to glow behind it

SUPER: LOOK CD4WARD

VIDEO: References fade into frame

SUPER: Reference list

VIDEO: RUKOBIA and ViiV logos fade in with trademark line; background music fades out

SUPER: For US Healthcare Professionals only. Trademarks are owned by or licensed to the ViiV Healthcare group of companies. ©2025 ViiV Healthcare or licensor. PMUS-FSTVID250005 May 2025 Produced in USA.

Temsavir, the active moiety of RUKOBIA, attaches directly to gp120 on the surface of HIV-1 virions, near the CD4⁺ attachment site. The attachment of temsavir locks gp120 into a closed formation that prevents the initial interaction between the virus and host immune cells.^1,4

This action prevents the first step of viral entry.³

Image of HIV-1 viron, CD4 T cell, gp120 and RUKOBIA

PREVENTS

RUKOBIA prevents HIV-1 attachment to CD4⁺ T-cells by binding to the gp120 subunit.^1,4

Image of HIV-1 viron, CD4 T cell, and RUKOBIA

PROTECTS

RUKOBIA protects uninfected CD4⁺ T-cells from HIV-1.¹

PRESERVES

RUKOBIA preserves CD4⁺ T-cell function by preventing viral replication.¹

Explore clinical evidence, including long-term data

Clinical Trial & Efficacy

ARV=antiretroviral; gp120=glycoprotein 120; HIV-1=human immunodeficiency virus type-1; MOA=mechanism of action.

References:

Ackerman P, Thompson M, Molina JM, et al. Long-term efficacy and safety of fostemsavir among subgroups of heavily treatment-experienced adults with HIV-1. AIDS 2021;35(7):1061-1072.
Lataillade M, Lalezari JP, Kozal M, et al. Safety and efficacy of the HIV-1 attachment inhibitor prodrug fostemsavir in heavily treatment-experienced individuals: week 96 results of the phase 3 BRIGHTE study. Lancet HIV. 2020;7(11):e740-e751. doi:10.1016/S2352-3018(20)30240-X
Cahn P, Fink V, Patterson P. Fostemsavir: a new CD4 attachment inhibitor. Curr Opin HIV AIDS. 2018;13(4):341-345.
Thompson M, Lalezari JP, Kaplan R, et al. Safety and efficacy of the HIV-1 attachment inhibitor prodrug fostemsavir in antiretroviral-experienced subjects: week 48 analysis of AI438011, a Phase IIb, randomized controlled trial. Antivir Ther. 2017;22(3):215-223.

PMUS-FSTWCNT250012

INDICATION

RUKOBIA, in combination with other antiretrovirals (ARVs), is indicated to treat HIV-1 infection in heavily treatment-experienced adults with multidrug-resistant HIV-1 infection failing their current ARV regimen due to resistance, intolerance, or safety considerations.

IMPORTANT SAFETY INFORMATION

Contraindications

Do not use in patients with previous hypersensitivity to fostemsavir or any of the components of RUKOBIA.
Do not use RUKOBIA in patients receiving strong cytochrome P450 (CYP)3A inducers, including but not limited to enzalutamide, carbamazepine, phenytoin, rifampin, mitotane, and St John’s wort (Hypericum perforatum).

INDICATION

IMPORTANT SAFETY INFORMATION

Contraindications

Do not use in patients with previous hypersensitivity to fostemsavir or any of the components of RUKOBIA.
Do not use RUKOBIA in patients receiving strong cytochrome P450 (CYP)3A inducers, including but not limited to enzalutamide, carbamazepine, phenytoin, rifampin, mitotane, and St John’s wort (Hypericum perforatum).

Warnings and precautions

Immune Reconstitution Syndrome, including the occurrence of autoimmune disorders with variable time to onset, has been reported with the use of RUKOBIA.

QTc Prolongation with Higher than Recommended Dosages: RUKOBIA at 2,400 mg twice daily has been shown to significantly prolong the QTc interval of the electrocardiogram. Use RUKOBIA with caution in patients with a history of QTc interval prolongation or in patients with relevant pre-existing cardiac disease or who are taking drugs with a known risk of Torsade de Pointes. Elderly patients may be more susceptible to drug-induced QT interval prolongation.

Elevations in Hepatic Transaminases in Patients with Hepatitis B or C Virus Co-infection:

Monitoring of liver chemistries is recommended in patients with hepatitis B and/or C co-infection.
Diligence should be applied in initiating or maintaining effective hepatitis B therapy when starting RUKOBIA in patients co-infected with hepatitis B.

Adverse Reactions or Loss of Virologic Response Due to Drug Interactions with concomitant use of RUKOBIA and other drugs may occur (see Contraindications and Drug Interactions).

Adverse reactions

The most common adverse reaction (all grades, randomized cohort) observed in ≥5% of subjects was nausea (10%).
81% of adverse reactions reported with RUKOBIA were mild or moderate in severity.

Drug interactions

See the full Prescribing Information for RUKOBIA for a complete list of significant drug interactions.
Temsavir may increase plasma concentrations of grazoprevir and voxilaprevir. Use an alternative hepatitis C virus regimen if possible.
Use the lowest possible starting dose for statins and monitor for statin-associated adverse events.
Patients receiving RUKOBIA should not take doses of estrogen-based therapies, including oral contraceptives, that contain more than 30 mcg/day of ethinyl estradiol. Caution is advised particularly in patients with additional risk factors for thromboembolic events.

Use in specific populations

Pregnancy: There are insufficient human data on the use of RUKOBIA during pregnancy to definitively assess a drug-associated risk for birth defects and miscarriage. An Antiretroviral Pregnancy Registry has been established.
Lactation: Potential risks of breastfeeding include HIV-1 transmission, developing viral resistance in HIV-positive infants, and adverse reactions in a breastfed infant.

Please see full Prescribing Information for RUKOBIA.

FSTWCNT230034

Trademarks are owned by or licensed to the ViiV Healthcare group of companies.

This site is funded and developed by ViiV Healthcare.

This site is intended for US healthcare professionals only.

PMUS-FSTWCNT250009 April 2025

Produced in USA.

Novel MOA: first and only ARV that inhibits HIV-1 attachment1,2

RUKOBIA is the only therapy that directly targets gp120 preventing attachment to protect CD4+ T-cells1,3

PREVENTS

PROTECTS

PRESERVES

Explore clinical evidence, including long-term data

INDICATION

IMPORTANT SAFETY INFORMATION

Contraindications

INDICATION

IMPORTANT SAFETY INFORMATION

Contraindications

Warnings and precautions

Adverse reactions

Drug interactions

Use in specific populations

Novel MOA: first and only ARV that inhibits HIV-1 attachment^1,2

RUKOBIA is the only therapy that directly targets gp120 preventing attachment to protect CD4⁺ T-cells^1,3